Lateral flow tests are used across a wide range of sectors including human health and pharma, environmental testing, animal health, food and feed testing, and plant and crop health. They are paper-based biosensors that fulfil all the demands of the World Health Organization for devices: the ASSURED criteria require them to be affordable, sensitive, selective, user-friendly, rapid and robust and derivable to the end-user. Paradoxically, sensitivity is not always assured.
Their way of working is simple: a fluid sample, with or without a specific analyte, is put in one end of the strip. Certain particles (transducers) prepared to attach to that analyte are dragged along by the fluid. A large amount of antibodies are placed in the test line to retain the analyte marked with the transducers. In case the analyte is present in the sample, the test line will be coloured because of the transducers. Otherwise, the particles will continue their journey to the end of the strip.
Researchers from the ICN2, in collaboration with University of Girona, have found a way to increase remarkably the sensitivity of the test with only a slight increase in time. The research has been led by ICREA Prof. Arben Merkoçi, Group Leader of the ICN2 Nanobioelectronics and Biosensors Group, and counted with the participation of the ICN2 Advanced AFM Laboratory too, led by Dr Neus Domingo. The results have been published in Biosensors and Bioelectronics with Dr Daniel Quesada-González, now researcher at the spin-off Paperdropdx, as its first author.